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pRK5-Myc-PSK2 (1-416) (K57A)
(Plasmid #197124)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197124 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    Alan Hall
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5948
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAOK1 (aa1-416, K57A)
  • Alt name
    PSK2 (aa1-416, K57A)
  • Alt name
    KIAA1361 (aa1-416, K57A)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1248
  • Mutation
    Changed K 57 to A and deleted C-terminus
  • GenBank ID
    57551
  • Entrez Gene
    TAOK1 (a.k.a. DDIB, KFC-B, MAP3K16, MARKK, PSK-2, PSK2, TAO1, hKFC-B, hTAOK1)
  • Promoter SV40
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer GGTCCAACTGCACCTCGG
  • 3′ sequencing primer GCAATAAACAAGTTGGGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned by Jonathan Morris at KCL (subject to MTA with Addgene).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-Myc-PSK2 (1-416) (K57A) was a gift from Jonathan Morris (Addgene plasmid # 197124 ; http://n2t.net/addgene:197124 ; RRID:Addgene_197124)

  • For your References section:

    Prostate-derived sterile 20-like kinases (PSKs/TAOKs) phosphorylate tau protein and are activated in tangle-bearing neurons in Alzheimer disease. Tavares IA, Touma D, Lynham S, Troakes C, Schober M, Causevic M, Garg R, Noble W, Killick R, Bodi I, Hanger DP, Morris JD. J Biol Chem. 2013 May 24;288(21):15418-29. doi: 10.1074/jbc.M112.448183. Epub 2013 Apr 12. 10.1074/jbc.M112.448183 PubMed 23585562
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