pRK5-Myc-PSK2 (1-416)
(Plasmid
#197123)
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PurposeExpression of Myc-tagged PSK2 (aa1-416) / TAOK1 (aa1-416) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
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Backbone manufacturerAlan Hall
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5951
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAOK1 (aa1-416)
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Alt namePSK2 (aa1-416)
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Alt nameKIAA1361 (aa1-416)
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SpeciesH. sapiens (human)
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Insert Size (bp)1248
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MutationDeleted C-terminus
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GenBank ID57551
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Entrez GeneTAOK1 (a.k.a. DDIB, KFC-B, MAP3K16, MARKK, PSK-2, PSK2, TAO1, hKFC-B, hTAOK1)
- Promoter SV40
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Tag
/ Fusion Protein
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer GGTCCAACTGCACCTCGG
- 3′ sequencing primer GCAATAAACAAGTTGGGC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned by Jonathan Morris at KCL (subject to MTA with Addgene).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5-Myc-PSK2 (1-416) was a gift from Jonathan Morris (Addgene plasmid # 197123 ; http://n2t.net/addgene:197123 ; RRID:Addgene_197123) -
For your References section:
Prostate-derived sterile 20-like kinases (PSKs/TAOKs) phosphorylate tau protein and are activated in tangle-bearing neurons in Alzheimer disease. Tavares IA, Touma D, Lynham S, Troakes C, Schober M, Causevic M, Garg R, Noble W, Killick R, Bodi I, Hanger DP, Morris JD. J Biol Chem. 2013 May 24;288(21):15418-29. doi: 10.1074/jbc.M112.448183. Epub 2013 Apr 12. 10.1074/jbc.M112.448183 PubMed 23585562