pBpaRS3tRNA
(Plasmid #197102)

• Purpose: To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.
• Depositing Lab: Kensaku Sakamoto
• Publication: Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. doi: 10.1021/bi1016183. Epub 2010 Dec 16.

Backbone

• Vector backbone: pACYC184
• Backbone manufacturer: MoBiTec GmbH, Germany
• Total vector size (bp): 3800
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TyrRS variant, amber suppressor tRNA
• Species: Methanocaldococcus jannaschii (TyrRS, tRNA)
• Mutation: Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (TyrRS with promoter), SphI(tRNA with promoter) (unknown if destroyed)
• 3′ cloning site: BglII (TyrRS), SalI (tRNA with terminator) (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry