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Addgene

pCDF-Mm2
(Plasmid #197100)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197100 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF-1b
  • Backbone manufacturer
    Novagen/Sigma Aldrich
  • Total vector size (bp) 2500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PylRS variant, Pyl tRNA, kanamycin resistance gene
  • Species
    Methanosarcina mazei (PylRS, tRNA), Bacteriai (Kanamycin)
  • Mutation
    Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF-Mm2 was a gift from Kensaku Sakamoto (Addgene plasmid # 197100 ; http://n2t.net/addgene:197100 ; RRID:Addgene_197100)

  • For your References section:

    Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates. Yamaguchi A, Matsuda T, Ohtake K, Yanagisawa T, Yokoyama S, Fujiwara Y, Watanabe T, Hohsaka T, Sakamoto K. Bioconjug Chem. 2016 Jan 20;27(1):198-206. doi: 10.1021/acs.bioconjchem.5b00602. Epub 2015 Dec 16. 10.1021/acs.bioconjchem.5b00602 PubMed 26625213
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