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PB-tetO-hNIL
(Plasmid #197089)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197089 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NGN2-P2A-ISL1-T2A-LHX3
  • Alt name
    NIL
  • Species
    H. sapiens (human)
  • Entrez Gene
    NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
  • Entrez Gene
    ISL1 (a.k.a. ISLET1, Isl-1)
  • Entrez Gene
    LHX3 (a.k.a. CPHD3, LIM3, M2-LHX3)
  • Promoter TRE3G

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-tetO-hNIL was a gift from Brian Wainger (Addgene plasmid # 197089 ; http://n2t.net/addgene:197089 ; RRID:Addgene_197089)

  • For your References section:

    iPSC motor neurons, but not other derived cell types, capture gene expression changes in postmortem sporadic ALS motor neurons. Held A, Adler M, Marques C, Reyes CJ, Kavuturu AS, Quadros ARAA, Ndayambaje IS, Lara E, Ward M, Lagier-Tourenne C, Wainger BJ. Cell Rep. 2023 Aug 30;42(9):113046. doi: 10.1016/j.celrep.2023.113046. 10.1016/j.celrep.2023.113046 PubMed 37651231
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