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Addgene

pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro
(Plasmid #197067)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197067 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSH-EFIRES-P
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MitoTrap-mCherry
  • GenBank ID

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #129715 was used in the creation of this plasmid. Mitotrap was derived from Addgene plasmid #46942.
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro was a gift from Julia Mahamid (Addgene plasmid # 197067 ; http://n2t.net/addgene:197067 ; RRID:Addgene_197067)

  • For your References section:

    Genetically encoded multimeric tags for subcellular protein localization in cryo-EM. Fung HKH, Hayashi Y, Salo VT, Babenko A, Zagoriy I, Brunner A, Ellenberg J, Muller CW, Cuylen-Haering S, Mahamid J. Nat Methods. 2023 Dec;20(12):1900-1908. doi: 10.1038/s41592-023-02053-0. Epub 2023 Nov 6. 10.1038/s41592-023-02053-0 PubMed 37932397
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