pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast
(Plasmid #197066)

• Purpose: Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP
• Depositing Lab: Julia Mahamid
• Publication: Fung et al Nat Methods. 2023 Dec;20(12):1900-1908. doi: 10.1038/s41592-023-02053-0. Epub 2023 Nov 6.

Backbone

• Vector backbone: pSH-EFIRES-B
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Encapsulin
• Alt name: GEM2
• Species: Synechococcus elongatus
• Mutation: Codon-optimised for human cell expression
• GenBank ID:
• Tags / Fusion Proteins:
  • HaloTag (C terminal on insert)
  • FKBP (C terminal on insert)

Resource Information

• Supplemental Documents:
  • 197066_J9V_8_p988.gbk
• A portion of this plasmid was derived from a plasmid made by: Plasmid includes DNA fragments derived from Addgene plasmid #129719.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.12.10.519870v1 for bioRxiv preprint.