pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1
(Plasmid
#197061)
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PurposeDonor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSH-EFIRES-P
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Vector typeMammalian Expression, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameEncapsulin
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Alt nameGEM2
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SpeciesSynechococcus elongatus
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MutationCodon-optimised for human cell expression
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GenBank ID
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Tags
/ Fusion Proteins
- HaloTag (C terminal on insert)
- FRB (C terminal on insert)
Gene/Insert 2
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Gene/Insert nameAdaptor Protein
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Alt nameFKBP-SNAP-vhhGFP4
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmids #129715 and #60510 were used in the creation of this plasmid. Anti-GFP nanobody vhhGFP4 was derived from Addgene #117713.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.12.10.519870v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1 was a gift from Julia Mahamid (Addgene plasmid # 197061 ; http://n2t.net/addgene:197061 ; RRID:Addgene_197061) -
For your References section:
Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM. Fung HKH, Hayashi Y, Salo VT, Babenko A, Zagoriy I, Brunner A, Ellenberg J, Müller CW, Cuylen-Haering S, Mahamid J. bioRxiv 2022.12.10.519870 10.1101/2022.12.10.519870