Skip to main content
Addgene

pgLAP5
(Plasmid #19706)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEF5/FRT-V5
  • Backbone size w/o insert (bp) 8365
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pgLAP5
  • Tags / Fusion Proteins
    • S peptide (C terminal on backbone)
    • Precission Protease site (C terminal on backbone)
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (not destroyed)
  • 3′ cloning site Gateway (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer TCC AGG GTC AAG GAA GGC ACG G
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pgLAP5 was a gift from Peter Jackson (Addgene plasmid # 19706 ; http://n2t.net/addgene:19706 ; RRID:Addgene_19706)
Related items:
Commonly requested with: