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pcDNA3.1_GEM4-Halo-FRB
(Plasmid #197057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197057 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Encapsulin
  • Alt name
    GEM4
  • Species
    Paraburkholderia xenovorans
  • Mutation
    Codon-optimised for human cell expression
  • GenBank ID
  • Tags / Fusion Proteins
    • HaloTag (C terminal on insert)
    • FRB (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_GEM4-Halo-FRB was a gift from Julia Mahamid (Addgene plasmid # 197057 ; http://n2t.net/addgene:197057 ; RRID:Addgene_197057)

  • For your References section:

    Genetically encoded multimeric tags for subcellular protein localization in cryo-EM. Fung HKH, Hayashi Y, Salo VT, Babenko A, Zagoriy I, Brunner A, Ellenberg J, Muller CW, Cuylen-Haering S, Mahamid J. Nat Methods. 2023 Dec;20(12):1900-1908. doi: 10.1038/s41592-023-02053-0. Epub 2023 Nov 6. 10.1038/s41592-023-02053-0 PubMed 37932397
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