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Addgene

yWL229 MCP-GFP-eRF3
(Plasmid #197053)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197053 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUbC
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pubc-nls-ha-MCP-GFP-eRF3
  • Species
    H. sapiens (human)
  • Promoter ubc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer ubc
  • 3′ sequencing primer WPRE
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    yWL229 MCP-GFP-eRF3 was a gift from Robert Singer (Addgene plasmid # 197053 ; http://n2t.net/addgene:197053 ; RRID:Addgene_197053)

  • For your References section:

    An improved imaging system that corrects MS2-induced RNA destabilization. Li W, Maekiniemi A, Sato H, Osman C, Singer RH. Nat Methods. 2022 Dec;19(12):1558-1562. doi: 10.1038/s41592-022-01658-1. Epub 2022 Nov 10. 10.1038/s41592-022-01658-1 PubMed 36357695
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