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Addgene

pWL078 MCP-2xGFP
(Plasmid #197047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS305
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MCP-2xGFP
  • Species
    S. cerevisiae (budding yeast)
  • Promoter CYC1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer CYC1
  • 3′ sequencing primer CYC1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWL078 MCP-2xGFP was a gift from Robert Singer (Addgene plasmid # 197047 ; http://n2t.net/addgene:197047 ; RRID:Addgene_197047)

  • For your References section:

    An improved imaging system that corrects MS2-induced RNA destabilization. Li W, Maekiniemi A, Sato H, Osman C, Singer RH. Nat Methods. 2022 Dec;19(12):1558-1562. doi: 10.1038/s41592-022-01658-1. Epub 2022 Nov 10. 10.1038/s41592-022-01658-1 PubMed 36357695
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