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Addgene

Atg24 1B
(Plasmid #197046)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197046 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET His6 TEV LIC cloning vector (1B)
  • Backbone size w/o insert (bp) 5340
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Atg24
  • Alt name
    SNX4
  • Alt name
    CVT13
  • Alt name
    Atg24
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1269
  • GenBank ID
    NM_001181470
  • Tag / Fusion Protein
    • 6His-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Atg24 1B was a gift from Michael Ragusa (Addgene plasmid # 197046 ; http://n2t.net/addgene:197046 ; RRID:Addgene_197046)

  • For your References section:

    A Comparative Analysis of the Membrane Binding and Remodeling Properties of Two Related Sorting Nexin Complexes Involved in Autophagy. Reinhart EF, Katzenell S, Andhare D, Bauer KM, Ragusa MJ. Biochemistry. 2022 Apr 14. doi: 10.1021/acs.biochem.2c00007. 10.1021/acs.biochem.2c00007 PubMed 35421303
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