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Addgene

ALFY-FYVE 1G
(Plasmid #197045)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197045 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET His6 GST TEV LIC cloning vector (1G)
  • Total vector size (bp) 6042
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ALFY
  • Alt name
    WDFY3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    225
  • Entrez Gene
    WDFY3 (a.k.a. ALFY, BCHS, MCPH18, ZFYVE25)
  • Tag / Fusion Protein
    • 6xHis-GST-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GST Forward
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ALFY-FYVE 1G was a gift from Michael Ragusa (Addgene plasmid # 197045 ; http://n2t.net/addgene:197045 ; RRID:Addgene_197045)

  • For your References section:

    A highly conserved glutamic acid in ALFY inhibits membrane binding to aid in aggregate clearance. Reinhart EF, Litt NA, Katzenell S, Pellegrini M, Yamamoto A, Ragusa MJ. Traffic. 2021 Jan;22(1-2):23-37. doi: 10.1111/tra.12771. Epub 2020 Dec 1. 10.1111/tra.12771 PubMed 33225481
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