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pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA
(Plasmid #197044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBT378
  • Backbone size w/o insert (bp) 6249
  • Total vector size (bp) 9446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    pCAG-FSF-LSL
  • Species
    S. cerevisiae (budding yeast); P1 bacteriophage
  • Insert Size (bp)
    933
  • Promoter pCAG

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    SP-HA-HRPtm
  • Species
    Synthetic
  • Insert Size (bp)
    1215
  • Promoter pCAG
  • Tag / Fusion Protein
    • HA, myc

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    WPRE-bGHpolyA
  • Species
    Synthetic; Woodchuck Hepatitis Virus
  • Insert Size (bp)
    797
  • Promoter pCAG

Cloning Information for Gene/Insert 3

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA was a gift from Liqun Luo (Addgene plasmid # 197044 ; http://n2t.net/addgene:197044 ; RRID:Addgene_197044)

  • For your References section:

    In situ cell-type-specific cell-surface proteomic profiling in mice. Shuster SA, Li J, Chon U, Sinantha-Hu MC, Luginbuhl DJ, Udeshi ND, Carey DK, Takeo YH, Xie Q, Xu C, Mani DR, Han S, Ting AY, Carr SA, Luo L. Neuron. 2022 Dec 7;110(23):3882-3896.e9. doi: 10.1016/j.neuron.2022.09.025. Epub 2022 Oct 10. 10.1016/j.neuron.2022.09.025 PubMed 36220098
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