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pGAD424-Rabex-5
(Plasmid #197043)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197043 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGAD424
  • Backbone size w/o insert (bp) 6665
  • Total vector size (bp) 8189
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rabex-5
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    1527
  • Entrez Gene
    RABGEF1 (a.k.a. RABEX5)
  • Promoter ADH1
  • Tag / Fusion Protein
    • GAL4 transcriptional activation domain (AD) fragment (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (destroyed during cloning)
  • 3′ cloning site BamH1 (destroyed during cloning)
  • 5′ sequencing primer GAL4 transcriptional activation domain forward
  • 3′ sequencing primer GAL4 transcriptional activation domain reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NotI/SpeI fragment encoding Rabex-5 was polished wIth T4 DNA polymerse and subcloned into pGAD424 digested with BamH1 and also polished wIth T4 DNA polymerase. Insert contains an additional N-terminal GRSSP sequence preceding the Rabex-5 initiation codon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGAD424-Rabex-5 was a gift from Juan Bonifacino (Addgene plasmid # 197043 ; http://n2t.net/addgene:197043 ; RRID:Addgene_197043)

  • For your References section:

    The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain. Mattera R, Tsai YC, Weissman AM, Bonifacino JS. J Biol Chem. 2006 Mar 10;281(10):6874-83. doi: 10.1074/jbc.M509939200. Epub 2006 Jan 5. 10.1074/jbc.M509939200 PubMed 16407276
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