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Addgene

pgLAP3
(Plasmid #19704)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF5/FRT-V5
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 8325
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pgLAP3
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • TEV (N terminal on backbone)
    • S peptide (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (not destroyed)
  • 3′ cloning site Gateway (not destroyed)
  • 5′ sequencing primer AGA AAC CGC TGC TGC TAA
  • 3′ sequencing primer TAG AAG GCA CAG TCG AGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pgLAP3 was a gift from Peter Jackson (Addgene plasmid # 19704 ; http://n2t.net/addgene:19704 ; RRID:Addgene_19704)
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