FSW-ALDH2-E504K-T2A-EGFP
(Plasmid
#197037)
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PurposeExpress ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197037 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameALDH2-E504K
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SpeciesH. sapiens (human)
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MutationE504K
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FSW-ALDH2-E504K-T2A-EGFP was a gift from Ronald Hart (Addgene plasmid # 197037 ; http://n2t.net/addgene:197037 ; RRID:Addgene_197037) -
For your References section:
Chronic alcohol metabolism results in DNA repair infidelity and cell cycle-induced senescence in neurons. Sun JK, Wu D, Wong GC, Lau TM, Yang M, Hart RP, Kwan KM, Chan HYE, Chow HM. Aging Cell. 2023 Feb;22(2):e13772. doi: 10.1111/acel.13772. Epub 2023 Jan 23. 10.1111/acel.13772 PubMed 36691110