pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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GenBank

SnapGene

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> Addgene NGS Result TATCCCCTGATTCTCTCGAGTTAACCCTAGAAAGATAATCATATTGTGACGTACGTTAAAGATAATCATG CGTAAAATTGACGCATGTGTTTTATCGATCTGTATATCGAGGTTTATTTATTAATTTGAATAGATATTAA GTTTTATTATATTTACACTTACATACTAATAATAAATTCAACAAACAATTTATTTATGTTTATTTATTTA TTAAAAAAAAACAAAAACTCAAAATTTCTTCTATAAAGTAACAAAACTTTTAAACATTCTCTCTTTTACA AAAATAAACTTATTTTGTACTTTAAAAACAGTCATGTTGTATTATAAAATAAGTAATTAGCTTAACTTAT ACATAATAGAAACAAATTATACTTATTAATTAATCTAGAGGTACCCGTTACATAACTTACGGTAAATGGC CCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAGTAACGCCAATAGGGACTTTCCATT GACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAG TACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTGTGCCCAGTACATGACCTTATGG GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGT TCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATT TTGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCRGGSGGGGSGGGGSGGGGSGGGGGGSGG GGSGGGGSGRGGSGGRGRGGKGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTTATGG CGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGCGCGCTGCCT TCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCA CAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCTGAGCAAGAGGTAAGGGTTTAAGG GATGGTTGGTTGGTGGGGTATTAATGTTTAATTACCTGGAGCACCTGCCTGAAATCACTTTTTTTCAGGT TGGACCGGTGCCACCATGGACTATAAGGACCACGACGGAGACTACAAGGATCATGATATTGATTACAAAG ACGATGACGATAAGATGCCTAAGAAGAAGAGAAAGGTGATGGGAAAGGGCGTGCTGGCCAAGGTGATGAA ATACGAGCTGAGATACCTGGATGGTTGTGGCGACTTCAGCAATATGCAGGAGCAGGTGTGGGCCCTGCAG CGGCAGACACGGGAAATCCTGAACAGATCCATCCAAATCGCCTTCCAATGGCGCTGCGCCAACAGCGAGC ACCACAGAAAGACCGGCGAGTACCTGGACCTGAAAACGGAAACCGGCTACAAGAGACTGGATGGCCACAT CTACAACTGCCTGAAGGGCCAGTACGAGGACATGGCCACATCTAACCTGAACGCCACCATCCAGAAGGCT TGGAAGAAGTATAACTCCAGCAAGAAGGAAATCCTGAGGGGCAGCATGAGCATCCCCAGCTACAAGATGA ACCAGCCTCTGCGACTGGACAAGAATACCGTGAAACTGTCTGAGGGCGAGCGGAACCCAATCGTGACCCT GACACTGTTTAGCGACAAGTTCAAGCGGGCCCAGGGCGTGTCCAACGTGAAGTTTAGCATGCCTCTGCAC GACGGCACCCAGAGAGCCATCTTCGCCAACCTGATGAACGGCACCTACCAGCTGGGAGAGTGCCAGCTGG TGTACAAACGGCCTAAGTGGTTCCTGTTCGTGACATACAAGTTCCCCCCCGTGGAACATCCTCTCGATCC TGACAAGATTCTGGGCGTCGCCATGGGCGAGGCCTGCGCGCTTTATGCCTCTACATTCGGCGAGCACGGC TACCTGAAGATCGATGGAGGCGAGATTACAAAGTACGCCAAGAAGATGGAAGCTAGAATCCGGAGCATGC AGAAGCAGGCTGCTCACTGTGGCGAAGGCAGAATCGGGCACGGCACCAAAACAAGAGTGTCTGTGGTGTA CCAGGCCAAGGACAAGGTGGCCAGATTCAGAGATACCATCAACCACAGATACTCTAAGGCCCTGATCGAC TACGCCCTGAAGAACCAGTGTGGCACCATCCAGATGGAAGATCTGACCGGCATCAAGGAAGATACAGGAT TTCCAAAGTTCCTGAGACATTGGACCTACTACGACCTGCAGAGCAAGATCGAGGCTAAGGCCGCCGAGCA CGGCATCCAAGTTGTCAAGATCAACCCTAGACACACCAGCCAGCGCTGCAGCAGATGTGGACACATCGAC AAAGCCAATAGAACCAGCCAAGCTGATTTCTGCTGCACCAAGTGCGGCTTCAGCGCCAATGCCGCCTTTA ATGCCAGCCAGAACATCAGCATCAGAAACATCGACAAGATTATCGCCAAGGCTATCGGCGCCAACCGGAA GCAGACCAAAAGGCCGGCGGCCACGAAGAAGGCCGGCCAGGCAAAGAAGAAGAAGGCCTATCCCTATGAC GTGCCCGATTATGCCAGCCTGGGCAGCGGCGACGGCATTGGTAGTGGGAGCAACGGCAGCAGCCTCGATG CTTTAGACGATTTTGACTTAGATATGCTTGGTTCAGACGCGTTAGACGACTTCGACCTAGACATGTTAGG CTCAGATGCATTGGACGACTTCGATTTAGATATGTTGGGCTCCGATGCCCTAGATGACTTTGATCTAGAT ATGCTAGGTAGTGGCGGCAGCGGATCCCAGTATCTGCCCGACACAGATGATAGACACCGAATCGAAGAGA AACGCAAGCGAACGTATGAAACCTTCAAATCGATCATGAAGAAATCGCCCTTCTCGGGTCCGACCGATCC CAGGCCCCCACCGAGAAGGATTGCGGTCCCGTCCCGCTCGTCGGCCAGCGTGCCGAAGCCTGCGCCGCAG CCCTACCCCTTCACGTCGAGCCTGAGCACAATCAATTATGACGAGTTCCCGACGATGGTGTTCCCCTCGG GACAAATCTCACAAGCCTCGGCGCTCGCACCAGCGCCTCCCCAAGTCCTTCCGCAAGCGCCTGCCCCAGC GCCTGCACCGGCAATGGTGTCCGCCCTCGCACAGGCCCCTGCGCCCGTCCCCGTGCTCGCGCCTGGACCG CCCCAGGCGGTCGCTCCACCGGCTCCGAAGCCGACGCAGGCCGGAGAGGGAACACTCTCCGAAGCACTTC TTCAACTCCAGTTTGATGACGAGGATCTTGGAGCACTCCTTGGAAACTCGACAGACCCTGCGGTGTTTAC CGACCTCGCGTCAGTAGATAACTCCGAATTTCAGCAGCTTTTGAACCAGGGTATCCCGGTCGCGCCACAT ACAACGGAGCCCATGTTGATGGAATACCCCGAAGCAATCACGAGACTTGTGACGGGAGCGCAGCGGCCTC CCGATCCCGCACCCGCACCTTTGGGGGCACCTGGCCTCCCTAACGGACTTTTGAGCGGCGACGAGGATTT CTCCTCCATCGCCGATATGGATTTCTCAGCCTTGCTGTCACAGATTTCCAGCGGCTCTGGCAGCGGCAGC CGGGATTCCAGGGAAGGGATGTTTTTGCCGAAGCCTGAGGCCGGCTCCGCTATTAGTGACGTGTTTGAGG GCCGCGAGGTGTGCCAGCCAAAACGAATCCGGCCATTTCATCCTCCAGGAAGTCCATGGGCCAACCGCCC ACTCCCCGCCAGCCTCGCACCAACACCAACCGGTCCAGTACATGAGCCAGTCGGGTCACTGACCCCGGCA CCAGTCCCTCAGCCACTGGATCCAGCGCCCGCAGTGACTCCCGAGGCCAGTCACCTGTTGGAGGATCCCG ATGAAGAGACGAGCCAGGCTGTCAAAGCCCTTCGGGAGATGGCCGATACTGTGATTCCCCAGAAGGAAGA GGCTGCAATCTGTGGCCAAATGGACCTTTCCCATCCGCCCCCAAGGGGCCATCTGGATGAGCTGACAACC ACACTTGAGTCCATGACCGAGGATCTGAACCTGGACTCACCCCTGACCCCGGAATTGAACGAGATTCTGG ATACCTTCCTGAACGACGAGTGCCTCTTGCATGCCATGCATATCAGCACAGGACTGTCCATCTTCGACAC ATCTCTGTTTCATATGGGTGGAGGCTCCGGGGAAGATCCTGCTGCCAAACGCGTTAAACTAGACATGGGA AGCGGAGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA TGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCAC CCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAG TTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCC CCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGATGACGGCGGCGTGGTGACCGTGACCCAGGACTC CTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCC GTAATGCAGAAGAAAACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGA AGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTA CAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCAC AACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACG AGCTGTACAAGTAAGAATTCCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCT GTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAA ATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAG CAAGGGGGAGGATTGGGAAGAGAATAGCAGGCATGCTGGGGAGAGGGCCTATTTCCCATGATTCCTTCAT ATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTGGAATTAATTTGACTGTAAACACAAAGATATT AGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGTTTGCAGTTTTAAAATTATGTTTTAAA ATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAAAG GACGAAACACCGAGGGCCGACTTCCCGGCCCAAAATCGAGACAGTAGCCGTAAAACGTTGAGTTTCAGCG TGGGCGACACACTCGAAAAGGTTAAGATATGCACATAGTAATCCGTGCATGAGCCGCGAAAGCGGCTTGA AGGTCTATCACTGATAGGGAGTATTTTATTTTTTTGCGGCCGCGTCGACGGCGCGCCCTAGACGAAGCAG CTCCAGCCTACACAATCGCTCAAGACGTGTAATGCTTTTATTATATATTAGTCACGATATCTATAACAAG AAAATATATATATAATAAGTTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAA ATCTTAAAAGTCACGTAAAAGATAATCATGCGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATCAG TGACACTTACCGCATTGACAAGCACGCCTCACGGGAGCTCCAAGCGGCGACTGAGATGTCCTAAATGCAC AGCGACGGATTCGCGCTATTTAGAAAGAGAGAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGA CTATCTTTCTAGGGTTAAGGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCTAG ACGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGG TAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTA CGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTT GACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAAC GGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGA GGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAGCATGAGCGAGCTGATTAAGGAGAA CATGCACATGAAGCTGTATATGGAGGGCACCGTGGACAACCATCACTTCAAGTGCACATCCGAGGGCGAA GGCAAGCCCTACGAGGGCACCCAGACCATGAGAATCAAGGTGGTCGAGGGCGGCCCTCTCCCCTTCGCCT TCGACATCCTGGCTACTAGCTTCCTCTACGGCAGCAAGACCTTCATCAACCACACCCAGGGCATCCCCGA CTTCTTCAAGCAGTCCTTCCCTGAGGGCTTCACATGGGAGAGAGTCACCACATACGAGGACGGGGGCGTG CTGACCGCTACCCAGGACACCAGCCTCCAGGACGGCTGCCTCATCTACAACGTCAAGATCAGAGGGGTGA ACTTCACATCCAACGGCCCTGTGATGCAGAAGAAAACACTCGGCTGGGAGGCCTTCACCGAGACACTGTA CCCCGCTGACGGCGGCCTGGAAGGCAGAAACGACATGGCCCTGAAGCTCGTGGGCGGGAGCCATCTGATC GCAAACATCAAGACCACATATAGATCCAAGAAACCCGCTAAGAACCTCAAGATGCCTGGCGTCTACTATG TGGACTACAGACTGGAAAGAATCAAGGAGGCCAACAACGAGACATACGTCGAGCAGCACGAGGTGGCAGT GGCCAGATACTGCGACCTCCCTAGCAAACTGGGGCACAAGCTGAATTAACTGTGCCTTCTAGTTGCCAGC CATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTA ATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAG GACAGCAAGGGGGAGGATTGGGAAGAGAATAGCAGGCATGCTGGGGAGGATCCGCACTTTTCGGGGAAAT GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAAC CCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTAT TCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTT TTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCG TATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGA GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCA CAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTA CTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTC GGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTA TGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCA AGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATC CTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAG AAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTC AGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTG TAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTG TCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCG TGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAA GCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCG CACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTT GAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTT TACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGT

pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA Sequences (1)

Full Sequences from Addgene (1)

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