AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR
(Plasmid #197029)

• Purpose: AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
• Depositing Lab: Xiangfeng Kong
• Publication: Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi: 10.1038/s41467-023-37829-7.

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Backbone

• Vector backbone: AAV2
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: humanized ecDHFR-enOsCas12f1-ecDHFR
• Species: Synthetic
• Mutation: D52R / T132R
• Promoter: MHCK7, hU6

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry