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Addgene

pCDNA4/TO-GFP-SPOP (R221C)
(Plasmid #197009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO/myc
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPOP
  • Species
    M. musculus (mouse)
  • Mutation
    R221C
  • Entrez Gene
    Spop (a.k.a. Pcif1, TEF2)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA4/TO-GFP-SPOP (R221C) was a gift from Tanja Mittag (Addgene plasmid # 197009 ; http://n2t.net/addgene:197009 ; RRID:Addgene_197009)

  • For your References section:

    Reduction of oligomer size modulates the competition between cluster formation and phase separation of the tumor suppressor SPOP. Sabri N, Cuneo MJ, Marzahn MR, Lee J, Bouchard JJ, Gullulu O, Vaithiyalingam S, Borgia MB, Schmit J, Mittag T. J Biol Chem. 2023 Dec;299(12):105427. doi: 10.1016/j.jbc.2023.105427. Epub 2023 Nov 4. 10.1016/j.jbc.2023.105427 PubMed 37926283
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