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Addgene

pET28-His10Smt3-gamma-toxin
(Plasmid #197000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Gamma toxin
  • Species
    Kluyveromyces lactis
  • GenBank ID
    CAA25571
  • Tag / Fusion Protein
    • His10-Smt3 (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Shuman Lab, Sloan Kettering Institute, New York, NY, USA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-His10Smt3-gamma-toxin was a gift from Dragony Fu (Addgene plasmid # 197000 ; http://n2t.net/addgene:197000 ; RRID:Addgene_197000)

  • For your References section:

    Monitoring the 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) modification in eukaryotic tRNAs via the gamma-toxin endonuclease. Lentini JM, Ramos J, Fu D. RNA. 2018 May;24(5):749-758. doi: 10.1261/rna.065581.118. Epub 2018 Feb 13. 10.1261/rna.065581.118 PubMed 29440318
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