pXJ40-HA-Merlin I S518A
(Plasmid #19700)

• Depositing Lab: Filippo Giancotti
• Publication: Okada et al J Cell Biol. 2005 Oct 24. 171(2):361-71.

Backbone

• Vector backbone: pXJ40-HA
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Merlin I
• Alt name: Nf2 neurofibromatosis 2
• Species: M. musculus (mouse)
• Insert Size (bp): 1800
• Mutation: Serine 518 to Alanine
• GenBank ID: P46662
• Entrez Gene: Nf2 (a.k.a. merlin)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pXJ40-HA
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry