pGBT9-ubiquitin
(Plasmid
#196999)
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PurposeExpression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGBT9
- Backbone size w/o insert (bp) 5524
- Total vector size (bp) 5751
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameubiquitin
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SpeciesH. sapiens (human)
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Insert Size (bp)231
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Entrez GeneUBC (a.k.a. HMG20)
- Promoter ADH1
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Tag
/ Fusion Protein
- GAL4-DNA binding domain fragment (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer GAL4 BD forward
- 3′ sequencing primer GAL4 BD reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBT9-ubiquitin was a gift from Juan Bonifacino (Addgene plasmid # 196999 ; http://n2t.net/addgene:196999 ; RRID:Addgene_196999) -
For your References section:
The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain. Mattera R, Tsai YC, Weissman AM, Bonifacino JS. J Biol Chem. 2006 Mar 10;281(10):6874-83. doi: 10.1074/jbc.M509939200. Epub 2006 Jan 5. 10.1074/jbc.M509939200 PubMed 16407276