pAAV-sCAG-GluClβ.CreON
(Plasmid #196996)

• Purpose: Cre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.
• Depositing Lab: David Bennett
• Publication: Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0000000000002976.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4994
• Total vector size (bp): 7019
• Vector type: AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GluClβ -YFP
• Alt name: GluCl-beta -YFP
• Alt name: GluClv2.0 β YFP
• Species: C. elegans (nematode); c elegan
• Insert Size (bp): 2025
• Promoter: short CAG
• Tag / Fusion Protein:
  • YFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: GGcgcgccctacaccagggactcgggggt
• 3′ sequencing primer: GCTAGCCCACCATGGCCACCCCCT

Resource Information

• Supplemental Documents:
  • pAAV-sCAG-Glucl-beta-CreON.gb
• A portion of this plasmid was derived from a plasmid made by: Original construct for GluCl β (GluCl β v2.0 Opt β-GluClv2.0) were a gift from Henry Lester (Addgene plasmid 47542) (Frazier et al., 2013)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original GluClβ (GluCl β v2.0 Opt β-GluClv2.0) (Frazier et al., 2013, PMID: 23720773)
First use in Dorsal root ganglia (Weir et al., 2017, PMID: 28969375)