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pRB051_LEU2
(Plasmid #196993)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    costum
  • Backbone manufacturer
    The Center for Synthetic Regulatory Genetics at NYU Langone Health
  • Backbone size (bp) 13328
  • Vector type
    Mammalian Expression, Mouse Targeting, Cre/Lox, Synthetic Biology ; Yeast assembly vector for Big-IN payloads
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    TransforMax EPI300
  • Growth instructions
    Copy number induction is recommended for high yield preps. See Lucigen’s growth and copy number induction protocol for TransforMax EPI300 cells.
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB051_LEU2 was a gift from Matthew Maurano (Addgene plasmid # 196993 ; http://n2t.net/addgene:196993 ; RRID:Addgene_196993)

  • For your References section:

    Synthetic regulatory genomics uncovers enhancer context dependence at the Sox2 locus. Brosh R, Coelho C, Ribeiro-Dos-Santos AM, Ellis G, Hogan MS, Ashe HJ, Somogyi N, Ordonez R, Luther RD, Huang E, Boeke JD, Maurano MT. Mol Cell. 2023 Apr 6;83(7):1140-1152.e7. doi: 10.1016/j.molcel.2023.02.027. Epub 2023 Mar 16. 10.1016/j.molcel.2023.02.027 PubMed 36931273
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