pLP-PIGA3 (pLP305)
(Plasmid
#196992)
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Purpose(Empty Backbone) Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecostum
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Backbone manufacturerThe Center for Synthetic Regulatory Genetics at NYU Langone Health
- Backbone size (bp) 1934
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Vector typeMammalian Expression, Mouse Targeting, Cre/Lox, Synthetic Biology ; Recombinase-mediated cassette exchange (RMCE) Landing Pad
- Promoter EF1a
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Selectable markersPuromycin ; hmPIGA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.06.20.495832v3 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLP-PIGA3 (pLP305) was a gift from Matthew Maurano (Addgene plasmid # 196992 ; http://n2t.net/addgene:196992 ; RRID:Addgene_196992) -
For your References section:
Synthetic regulatory genomics uncovers enhancer context dependence at the Sox2 locus. Brosh R, Coelho C, Ribeiro-Dos-Santos AM, Ellis G, Hogan MS, Ashe HJ, Somogyi N, Ordonez R, Luther RD, Huang E, Boeke JD, Maurano MT. Mol Cell. 2023 Apr 6;83(7):1140-1152.e7. doi: 10.1016/j.molcel.2023.02.027. Epub 2023 Mar 16. 10.1016/j.molcel.2023.02.027 PubMed 36931273