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Addgene

pLP-PIGA3 (pLP305)
(Plasmid #196992)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196992 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    costum
  • Backbone manufacturer
    The Center for Synthetic Regulatory Genetics at NYU Langone Health
  • Backbone size (bp) 1934
  • Vector type
    Mammalian Expression, Mouse Targeting, Cre/Lox, Synthetic Biology ; Recombinase-mediated cassette exchange (RMCE) Landing Pad
  • Promoter EF1a
  • Selectable markers
    Puromycin ; hmPIGA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLP-PIGA3 (pLP305) was a gift from Matthew Maurano (Addgene plasmid # 196992 ; http://n2t.net/addgene:196992 ; RRID:Addgene_196992)
  • For your References section:

    Synthetic regulatory genomics uncovers enhancer context dependence at the Sox2 locus. Brosh R, Coelho C, Ribeiro-Dos-Santos AM, Ellis G, Hogan MS, Ashe HJ, Somogyi N, Ordonez R, Luther RD, Huang E, Boeke JD, Maurano MT. Mol Cell. 2023 Apr 6;83(7):1140-1152.e7. doi: 10.1016/j.molcel.2023.02.027. Epub 2023 Mar 16. 10.1016/j.molcel.2023.02.027 PubMed 36931273