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pCI-neo-(HA)3-ubiquitin
(Plasmid #196991)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196991 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 5788
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ubiquitin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    329
  • Mutation
    silent mutation in ubiquitin codon 13
  • Entrez Gene
    UBC (a.k.a. HMG20)
  • Promoter CMV
  • Tag / Fusion Protein
    • triple HA tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pCI-neo forward
  • 3′ sequencing primer pCI-neo reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

triple HA tag subcloned between XhoI and EcoRI sites of pCI-neo; ubiquitin subcloned between EcoRI and SalI sites of pCI-neo

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo-(HA)3-ubiquitin was a gift from Juan Bonifacino (Addgene plasmid # 196991 ; http://n2t.net/addgene:196991 ; RRID:Addgene_196991)

  • For your References section:

    The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain. Mattera R, Tsai YC, Weissman AM, Bonifacino JS. J Biol Chem. 2006 Mar 10;281(10):6874-83. doi: 10.1074/jbc.M509939200. Epub 2006 Jan 5. 10.1074/jbc.M509939200 PubMed 16407276
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