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pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP
(Plasmid #196988)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196988 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Total vector size (bp) 14387
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nestin-dCas9-KRAB-T2a-GFP

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.08.10.455866 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP was a gift from Elizabeth Kirby (Addgene plasmid # 196988 ; http://n2t.net/addgene:196988 ; RRID:Addgene_196988)

  • For your References section:

    Intracrine VEGF Signaling Is Required for Adult Hippocampal Neural Stem Cell Maintenance and Vascular Proximity. Dause TJ, Osap R, Kuwahara AA, Denninger JK, Kirby ED. Mol Neurobiol. 2025 Mar 25. doi: 10.1007/s12035-025-04861-1. 10.1007/s12035-025-04861-1 PubMed 40131696
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