Cas9 TLS2
(Plasmid #196978)

• Purpose: Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.
• Depositing Lab: Friedrich Kragler
• Publication: Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01585-8.

Backbone

• Vector backbone: pMDC7
• Total vector size (bp): 16797
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9 with TLS2 tag
• Species: Streptococcus pyogenes
• Insert Size (bp): 4323
• Promoter: pG10-90

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: GATCTTCGCAAGACCCTTCCTC
• 3′ sequencing primer: GAAACTGATGCATTGAACTTGACGAA

Resource Information

• Supplemental Documents:
  • cas9-tls2.gb
  • GO CRISP Cloning Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please refer to the GO CRISP Cloning Protocol linked above for additional information.