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pET28a_DmKHC[1-421]-SNAP-6xHis
(Plasmid #196975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5212
  • Total vector size (bp) 7051
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Purification from BL21. Induce protein expression with IPTG. After induction, grow at 18 degrees C.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Kinesin Heavy Chain
  • Alt name
    KHC, Khc, kinesin-1
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1839
  • Entrez Gene
    Khc (a.k.a. Dmel_CG7765, 2R6, CG7765, DK, DKH, Dm KHC, DmK, DmKHC, Dmel\CG7765, Dmkin, KHC, KIF 5A, KIF5, KIF5B, KIN, Kif5, Kin, Kin-1, Kinesin, Kinesin-1, khc, kin, kinesin, kinesin-1, l(2)W12, l(2)k13219, l(2)k13314, l(2R)W12, pgs)
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • 6xHis (C terminal on insert)
    • SNAP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AATAATCCATGGGCATGAGCGCAGAACGAGAAATTCCAGC
  • 3′ sequencing primer GGGTTATGCTAGTTATTGCTCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a_DmKHC[1-421]-SNAP-6xHis was a gift from Lukas Kapitein (Addgene plasmid # 196975 ; http://n2t.net/addgene:196975 ; RRID:Addgene_196975)

  • For your References section:

    Direct observation of motor protein stepping in living cells using MINFLUX. Deguchi T, Iwanski MK, Schentarra EM, Heidebrecht C, Schmidt L, Heck J, Weihs T, Schnorrenberg S, Hoess P, Liu S, Chevyreva V, Noh KM, Kapitein LC, Ries J. Science. 2023 Mar 10;379(6636):1010-1015. doi: 10.1126/science.ade2676. Epub 2023 Mar 9. 10.1126/science.ade2676 PubMed 36893247
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