pPB-TREG3G-PE2-rtTA3G-P2A-eGFP
(Plasmid #196971)

• Purpose: Piggybac vector with doxycyclin-inducible prime editor for mammalian cells
• Depositing Lab: Leopold Parts
• Publication: Predicting efficiency of writing short sequences into the genome using prime editing (unpublished)

Backbone

• Vector backbone: pPB
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Cas9-RT
• Alt name: prime editor
• Insert Size (bp): 6321
• Promoter: TREG3G

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TREG3G-F

Gene/Insert 2

• Gene/Insert name: rtTA3G-P2A-eGFP
• Insert Size (bp): 1527
• Promoter: PGK

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: PGK-F

Resource Information

• Supplemental Documents:
  • pPB_TREG3G-PE2-rtTA3G-P2A-eGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry