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Addgene

pLentiGuide-BlastR
(Plasmid #196970)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196970 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiGuide-Puro(Addgene#:52963)
  • Backbone size (bp) 8103
  • Vector type
    CRISPR
  • Promoter hU6
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Only amplify in RecA- bacteria
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer hU6-F (5'-ggctttatatatcttgtggaaaggacgaaacacc-3')
  • 3′ sequencing primer hGata4-rev (5'-ATTGTGGATGAATACTGCC-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiGuide-BlastR was a gift from Leopold Parts (Addgene plasmid # 196970 ; http://n2t.net/addgene:196970 ; RRID:Addgene_196970)
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