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Addgene

pCMV-PE2-P2A-PuroR
(Plasmid #196962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Total vector size (bp) 10423
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2-P2A-Puro
  • Alt name
    Cas9-RT, Cas9(H840A)-M-MLVrt(D200N, T306K, W313F, T330P, L603W)-P2A-GFP
  • Insert Size (bp)
    6990
  • Promoter CMV

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7-forward
  • 3′ sequencing primer BGH-reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-PE2-P2A-PuroR was a gift from Leopold Parts (Addgene plasmid # 196962 ; http://n2t.net/addgene:196962 ; RRID:Addgene_196962)
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