pEGFP-CLCa
(Plasmid #196912)

• Purpose: Visualization of clathrin-coated pit formation in living mammalian cells
• Depositing Lab: Kenji Sugimoto
• Publication: Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2):571-4. doi: 10.1271/bbb.60434. Epub 2007 Feb 7.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5600
• Modifications to backbone: EcoR I and Sal I sites of pEGFP-ΔC2, which had been derived from pEGFP-C1 by cutting and filling-in Hind III site and converted to C2 reading frame
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Clathrin-Coated light chain
• Alt name: NM_007096
• Species: H. sapiens (human)
• Insert Size (bp): 900
• GenBank ID:
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: EGFP-C-ter
• 3′ sequencing primer: SV40 Poly A site

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Obtained by screening the human cDNA library (purchased from Origene).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

900bp of EcoRI-XhoI fragment of pJG4-5 clone y22-1, from OriGene human MCF7 cDNA Library, was recloned into EcoR I and Sal I sites of pEGFP-ΔC2, which had been derived from pEGFP-C1 by cutting and filling-in Hind III site and converted to C2 reading frame.