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Addgene

pHcRed-CLCa
(Plasmid #196911)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196911 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHcRed-Tandem-C1
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Clathrin-Coated light chain
  • Alt name
    NM_007096
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • HcRed (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer HcRed C-ter side
  • 3′ sequencing primer SV40 poly A side
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Obtained by screening the human cDNA library (purchased from Origene).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

900bp Sac II-Xho I fragment of pEGFP-ΔC2-y22-1 was recloned into the same site of pHcRed-Tandem-C1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHcRed-CLCa was a gift from Kenji Sugimoto (Addgene plasmid # 196911 ; http://n2t.net/addgene:196911 ; RRID:Addgene_196911)

  • For your References section:

    Dynamic behavior of FCHO1 revealed by live-cell imaging microscopy: its possible involvement in clathrin-coated vesicle formation. Sakaushi S, Inoue K, Zushi H, Senda-Murata K, Fukada T, Oka S, Sugimoto K. Biosci Biotechnol Biochem. 2007 Jul;71(7):1764-8. doi: 10.1271/bbb.60720. Epub 2007 Jul 7. 10.1271/bbb.60720 PubMed 17617719
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