pEGFP-FCHO1
(Plasmid #196910)

• Purpose: visualization of clathrin-coaed vesicle formation in living mammalian cells
• Depositing Lab: Kenji Sugimoto
• Publication: Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7):1764-8. doi: 10.1271/bbb.60720. Epub 2007 Jul 7.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FCHO1
• Alt name: KIAA0290
• Alt name: Fes/CIP4 homology (FCH) domain only 1
• Species: H. sapiens (human)
• Entrez Gene: FCHO1 (a.k.a. IMD76)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned gene (KIAA0290) was obtained from Kazusa (http://www.kazusa.or.jp/huge/).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

https://www.jstage.jst.go.jp/article/bbb/71/7/71_60720/_pdf/-char/en