pEGFP-survivin
(Plasmid #196908)

• Purpose: visualization of midzone on anaphase and midbody later during mitosis in living mammalian cells
• Depositing Lab: Kenji Sugimoto
• Publication: Fukada et al Biotechniques. 2004 Oct;37(4):552, 554, 556. doi: 10.2144/04374BM05.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5100
• Modifications to backbone: EcoR I and Sal I sites of pEGFP-ΔC2, which had been derived from pEGFP-C1 by cutting and filling-in Hind III site and converted to C2 reading frame
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: survivin
• Species: H. sapiens (human)
• Insert Size (bp): 400
• Mutation: E129K
• GenBank ID: NM_001168
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: OriGene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

400bp BamHI-EcoRI fragment of pcDNA3-survivin was inserted into Bgl II and EcoRI sites of EGFP-C1