pGC86
(Plasmid
#19690)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR-XL-TOPO
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Backbone manufacturerInvitrogen
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehlh-12(R25K)_genomic region
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SpeciesC. elegans (nematode)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGC86 was a gift from Jane Hubbard (Addgene plasmid # 19690 ; http://n2t.net/addgene:19690 ; RRID:Addgene_19690) -
For your References section:
A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Voutev R, Hubbard EJ. Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274. 10.1534/genetics.108.090274 PubMed 18723890