pFastBac Mel18 flag
(Plasmid
#1969)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1969 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFastBac
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4775
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Vector typeInsect Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMel18
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SpeciesM. musculus (mouse)
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Entrez GenePcgf2 (a.k.a. Mel1, Mel18, Rnf1, Rnf110, Zfp14, Zfp144, mel-1, mel-18)
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Tag
/ Fusion Protein
- flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mouse Mel-18 cDNA with C-terminal Flag fusion. (kingston #1386)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac Mel18 flag was a gift from Robert Kingston (Addgene plasmid # 1969 ; http://n2t.net/addgene:1969 ; RRID:Addgene_1969) -
For your References section:
Propagation of silencing; recruitment and repression of naive chromatin in trans by polycomb repressed chromatin. Lavigne M, Francis NJ, King IF, Kingston RE. Mol Cell 2004 Feb 13;13(3):415-25. 10.1016/S1097-2765(04)00006-1 PubMed 14967148
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/54/08/fbffd730-af60-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)