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Addgene

pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox
(Plasmid #196889)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196889 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-lox
  • Backbone size w/o insert (bp) 5742
  • Vector type
    Mammalian Expression, Cre/Lox ; Dre/Rox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lyn-mScarlet
  • Species
    Synthetic
  • Insert Size (bp)
    741
  • Promoter Quimeric CAG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pCAG forward (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer M13_ (-20) forward primer (5'-GTAAAACGACGGCCAGT-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Membrane targetting sequence is Lyn (MGCIKSKRKD)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox was a gift from Frank Bradke (Addgene plasmid # 196889 ; http://n2t.net/addgene:196889 ; RRID:Addgene_196889)

  • For your References section:

    Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, Bradke F. Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15. 10.1016/j.neuron.2023.01.020 PubMed 36796357
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