pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP
(Plasmid
#196875)
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PurposeNeuron-specific expression of LynGFP reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG-lox
- Backbone size w/o insert (bp) 7592
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLynEGFP
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SpeciesAequorea victoria
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Insert Size (bp)765
- Promoter Quimeric CAG
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pCAG forward (5'-GCAACGTGCTGGTTATTGTG-3')
- 3′ sequencing primer M13_ (-20) forward primer (5'-GTAAAACGACGGCCAGT-3') (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP was a gift from Frank Bradke (Addgene plasmid # 196875 ; http://n2t.net/addgene:196875 ; RRID:Addgene_196875) -
For your References section:
Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, Bradke F. Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15. 10.1016/j.neuron.2023.01.020 PubMed 36796357