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pCR-XL-rAkap9
(Plasmid #196867)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196867 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-XL-TOPO
  • Backbone size w/o insert (bp) 3519
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 30 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow 2-3 days at room temperature. See comments section for more details.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Akap9
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    11505
  • Entrez Gene
    Akap9 (a.k.a. CG-NAP, Gisp, Yotiao)
  • Promoter Lac promoter and T7 promoter

Cloning Information

  • Cloning method TOPO Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For successful transformation of Plasmid 196867: pCR-XL-rAkap9, it is recommended to use electroporation and to grow transformed bacteria at no more than 28 °C for two days and with 30 μg/ml kanamycin on LB plates for full length plasmid production

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR-XL-rAkap9 was a gift from Frank Bradke (Addgene plasmid # 196867 ; http://n2t.net/addgene:196867 ; RRID:Addgene_196867)

  • For your References section:

    Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, Bradke F. Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15. 10.1016/j.neuron.2023.01.020 PubMed 36796357
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