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pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD
(Plasmid #196856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBeta-actin-AcGFP-CI
  • Backbone size w/o insert (bp) 6748
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AcGFP-Cdk5rap2-CTDdeltaCBD
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    487
  • Mutation
    Lacking the centrosome-targeting domain (CTD)
  • Entrez Gene
    Cdk5rap2
  • Promoter Chicken bactin (plus Chicken bactin intron)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pCAG forward (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Lacking the centrosome-binding domain (CBD): VITHQVLRKA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD was a gift from Frank Bradke (Addgene plasmid # 196856 ; http://n2t.net/addgene:196856 ; RRID:Addgene_196856)

  • For your References section:

    Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, Bradke F. Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15. 10.1016/j.neuron.2023.01.020 PubMed 36796357
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