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Addgene

pBactin-mOrange2-CI
(Plasmid #196852)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196852 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBeta-actin
  • Backbone size w/o insert (bp) 6030
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mOrange2
  • Species
    Discosoma sp.
  • Insert Size (bp)
    734
  • Mutation
    None (wt)
  • Promoter Chicken bactin (plus Chicken bactin intron)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer pCAG forward (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pBeta-actin [Kapitein et al. , 2010]

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBactin-mOrange2-CI was a gift from Frank Bradke (Addgene plasmid # 196852 ; http://n2t.net/addgene:196852 ; RRID:Addgene_196852)

  • For your References section:

    Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, Bradke F. Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15. 10.1016/j.neuron.2023.01.020 PubMed 36796357
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