pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7
(Plasmid
#196846)
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Purpose(Empty Backbone) backbone for gRNA cloning of dpspCas13b tagged by 2xPP7
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCR8
- Backbone size (bp) 3308
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.06.21.497089v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7 was a gift from Albert Cheng (Addgene plasmid # 196846 ; http://n2t.net/addgene:196846 ; RRID:Addgene_196846) -
For your References section:
Simultaneous multifunctional transcriptome engineering by CRISPR RNA scaffold. Liu Z, Jillette N, Robson P, Cheng AW. Nucleic Acids Res. 2023 Aug 11;51(14):e77. doi: 10.1093/nar/gkad547. 10.1093/nar/gkad547 PubMed 37395412
Map uploaded by the depositor.
