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pOBD2 Pab1 E181R (123-204)
(Plasmid #196835)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196835 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOBD2
  • Backbone size w/o insert (bp) 7167
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pab1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    241
  • Mutation
    123-204 amino acids & E181R
  • Entrez Gene
    PAB1 (a.k.a. YER165W)
  • Promoter ADH
  • Tag / Fusion Protein
    • Gal4 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pOBD2 + NcoI-PAB1(369-612 bp)-SalI. Expresses Pab1 RRM2 fragment with E181R mutation. Expresses Pab1 fragment fused to GAL4BD.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOBD2 Pab1 E181R (123-204) was a gift from Stan Fields (Addgene plasmid # 196835 ; http://n2t.net/addgene:196835 ; RRID:Addgene_196835)

  • For your References section:

    Combining natural sequence variation with high throughput mutational data to reveal protein interaction sites. Melamed D, Young DL, Miller CR, Fields S. PLoS Genet. 2015 Feb 11;11(2):e1004918. doi: 10.1371/journal.pgen.1004918. eCollection 2015 Feb. 10.1371/journal.pgen.1004918 PubMed 25671604
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