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pOAD eIF4G1 (1-341)
(Plasmid #196833)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196833 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOAD
  • Backbone size w/o insert (bp) 8429
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eIF4G1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1023
  • Mutation
    amino acids 1-341
  • Entrez Gene
    TIF4631 (a.k.a. YGR162W)
  • Promoter ADH
  • Tag / Fusion Protein
    • Gal4 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pOAD + EcoRI-eIF4G1(1-1023)-SalI. (W303 strain as a genomic template). Expresses eIF4G1 N' terminus fused to GAL4AD

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOAD eIF4G1 (1-341) was a gift from Stan Fields (Addgene plasmid # 196833 ; http://n2t.net/addgene:196833 ; RRID:Addgene_196833)

  • For your References section:

    Combining natural sequence variation with high throughput mutational data to reveal protein interaction sites. Melamed D, Young DL, Miller CR, Fields S. PLoS Genet. 2015 Feb 11;11(2):e1004918. doi: 10.1371/journal.pgen.1004918. eCollection 2015 Feb. 10.1371/journal.pgen.1004918 PubMed 25671604
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