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pzk228-pmax-SgrAI[3xFLAG]-AscI-dPspCas13b(AAAA)
(Plasmid #196827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAC90-pmaxGW
  • Backbone size w/o insert (bp) 2955
  • Total vector size (bp) 6431
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dpspCas13b(AAAA)
  • Insert Size (bp)
    3474
  • Mutation
    K367A, D369A and K370A
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pzk228-pmax-SgrAI[3xFLAG]-AscI-dPspCas13b(AAAA) was a gift from Albert Cheng (Addgene plasmid # 196827 ; http://n2t.net/addgene:196827 ; RRID:Addgene_196827)

  • For your References section:

    Simultaneous multifunctional transcriptome engineering by CRISPR RNA scaffold. Liu Z, Jillette N, Robson P, Cheng AW. Nucleic Acids Res. 2023 Aug 11;51(14):e77. doi: 10.1093/nar/gkad547. 10.1093/nar/gkad547 PubMed 37395412
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