pOBD2
(Plasmid
#196826)
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Purpose(Empty Backbone) Yeast two-hybrid ; Gal4 DNA binding domain
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196826 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneYCplac22
- Backbone size (bp) 7167
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Vector typeYeast Expression
- Promoter ADH
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Selectable markersTRP1
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Tag
/ Fusion Protein
- Gal4 DNA binding domain (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOBD2 was a gift from Stan Fields (Addgene plasmid # 196826 ; http://n2t.net/addgene:196826 ; RRID:Addgene_196826) -
For your References section:
A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Uetz P, Giot L, Cagney G, Mansfield TA, Judson RS, Knight JR, Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li Y, Godwin B, Conover D, Kalbfleisch T, Vijayadamodar G, Yang M, Johnston M, Fields S, Rothberg JM. Nature. 2000 Feb 10;403(6770):623-7. doi: 10.1038/35001009. 10.1038/35001009 PubMed 10688190
